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Fig. 6 | BMC Microbiology

Fig. 6

From: Multi-factorial examination of amplicon sequencing workflows from sample preparation to bioinformatic analysis

Fig. 6

Compositional biases vary depending on the methodological factors used in each workflow. Correlation to the original mock community member estimated abundance was maximized when conducting 1-step PCR with the V4-V4 primer set and iTaq followed by denoising and read assembly with mothur. Data shown represent Spearman’s rank correlation between qPCR-derived approximate relative abundance of individuals originally included in the mock community to the relative abundance of exact sequence variants output from the bioinformatic processing programs

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BMC Microbiology

ISSN: 1471-2180

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