Table 4 ANOVA, regression analysis, and significance of the response surface methodology to optimize β-glucan biosynthesis by Meyerozyma guilliermondii
Run Order | Nitrogen source conc. (gl−1) | Phosphate source conc. (gl−1) | β-glucan Conc. (mg/100 ml) | ||||
|---|---|---|---|---|---|---|---|
1 | 7.00 (+ 1) | 6.00 (+ 1) | 657.1 ± 52.7 | ||||
2 | 3.85 (0) | 3.30 (0) | 514.0 ± 5.1 | ||||
3 | 0.70 (-1) | 6.00 (+ 1) | 1050.1 ± 54.1 | ||||
4 | 0.70 (-1) | 0.60 (-1) | 835.0 ± 30.9 | ||||
5 | 3.85 (0) | 3.30 (0) | 502.0 ± 17.1 | ||||
6 | 0.07 (-1.2) | 3.30 (0) | 770.6 ± 0.2 | ||||
7 | 7.00 (+ 1) | 6.00 (+ 1) | 762.3 ± 52.7 | ||||
8 | 0.70 (-1) | 0.60 (-1) | 773.2 ± 30.9 | ||||
9 | 3.85 (0) | 3.30 (0) | 523.4 ± 4.3 | ||||
10 | 3.85 (0) | 3.30 (0) | 533.0 ± 13.9 | ||||
11 | 3.85 (0) | 3.30 (0) | 528.7 ± 9.6 | ||||
12 | 3.85 (0) | 3.30 (0) | 502.0 ± 17.1 | ||||
13 | 3.85 (0) | 3.30 (0) | 514.0 ± 5.1 | ||||
14 | 3.85 (0) | 3.30 (0) | 523.4 ± 4.3 | ||||
15 | 3.85 (0) | 3.30 (0) | 533.0 ± 13.9 | ||||
16 | 3.85 (0) | 0.06 (-1.2) | 723.4 ± 40.6 | ||||
17 | 3.85 (0) | 6.54 (+ 1.2) | 827.0 ± 9.0 | ||||
18 | 3.85 (0) | 0.06 (-1.2) | 642.2 ± 40.6 | ||||
19 | 0.70 (-1) | 6.00 (+ 1) | 942.0 ± 54.1 | ||||
20 | 7.00 (+ 1) | 0.60 (-1) | 778.7 ± 56.2 | ||||
21 | 7.00 (+ 1) | 0.60 (-1) | 891.0 ± 56.2 | ||||
22 | 3.85 (0) | 6.54 (+ 1.2) | 809.0 ± 9.0 | ||||
23 | 7.63 (+ 1.2) | 3.30 (0) | 746.0 ± 0.5 | ||||
24 | 7.63 (+ 1.2) | 3.30 (0) | 747.0 ± 0.5 | ||||
25 | 3.85 (0) | 3.30 (0) | 517.5 ± 1.6 | ||||
26 | 0.07 (-1.2) | 3.30 (0) | 770.2 ± 0.2 | ||||
Model Summary | |||||||
Source | DF | Adj. SS | Adj. MS | F-Value | P-Value | Coefficient | T-Value |
Model | 5 | 572432 | 114486 | 50.99 | |||
Linear | 2 | 38590 | 19295 | 8.59 | 0.002 | ||
Nitrogen Conc | 1 | 15956 | 15956 | 7.11 | 0.015 | -40.6 | -2.67 |
Phosphate Conc | 1 | 22635 | 22635 | 10.08 | 0.005 | 48.7 | 3.18 |
Square | 2 | 466461 | 233230 | 103.88 | |||
Nitrogen*Nitrogen | 1 | 222414 | 222414 | 99.06 | < 0.001 | 226.9 | 9.95 |
Phosphate* Phosphate | 1 | 206915 | 206915 | 92.16 | < 0.001 | 218.9 | 9.60 |
2-Way Interaction | 1 | 67381 | 67381 | 30.01 | |||
Nitrogen*Phosphate | 1 | 67381 | 67381 | 30.01 | < 0.001 | -132.2 | -5.48 |
Error | 20 | 44903 | 2245 | ||||
Lack-of-Fit | 3 | 9517 | 3172 | 1.52 | 0.244 | ||
Pure Error | 17 | 35386 | 2082 | ||||
Total | 25 | 617335 | |||||