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Table 3 ANOVA, regression analysis, and significance of the response surface methodology to optimize β-glucan biosynthesis by Kluyveromyces lactis

From: Optimizing the synthesis of yeast Beta-glucan via response surface methodology for nanotechnology application

Run Order

Minerals conc. (ml/l)

Vitamins conc. (gl−1)

β-glucan Conc. (mg/100 ml)

1

1.50 (-1)

0.500 (+ 1)

324.5 ± 4.4

2

1.50 (-1)

0.050 (-1)

300.0 ± 5.0

3

8.25 (0)

0.275 (0)

403.0 ± 3.1

4

8.25 (0)

0.275 (0)

403.0 ± 3.1

5

8.25 (0)

0.275 (0)

431.0 ± 24.9

6

8.25 (0)

0.005 (-1.2)

322.1 ± 9.0

7

8.25 (0)

0.275 (0)

403.0 ± 4.4

8

8.25 (0)

0.545 (+ 1.2)

293.3 ± 29.4

9

1.50 (-1)

0.050 (-1)

290.5 ± 5.0

10

1.50 (-1)

0.500 (+ 1)

333.3 ± 4.4

11

8.25 (0)

0.275 (0)

403.0 ± 4.4

12

8.25 (0)

0.275 (0)

403.0 ± 4.4

13

8.25 (0)

0.275 (0)

403.0 ± 4.4

14

15.00 (+ 1)

0.050 (-1)

372.0 ± 6.0

15

0.15 (-1.2)

0.275 (0)

345.5 ± 2.8

16

8.25 (0)

0.275 (0)

403.0 ± 4.4

17

0.15 (-1.2)

0.275 (0)

340.0 ± 2.8

18

8.25 (0)

0.275 (0)

393.3 ± 12.8

19

16.35 (+ 1.2)

0.275 (0)

375.3 ± 5.7

20

8.25 (0)

0.005 (-1.2)

340.0 ± 9.0

21

15.00 (+ 1)

0.050 (-1)

360.0 ± 6.0

22

15.00 (+ 1)

0.500 (+ 1)

289.1 ± 6.5

23

16.35 (+ 1.2)

0.275 (0)

364.0 ± 5.7

24

8.25 (0)

0.275 (0)

416.0 ± 9.9

25

8.25 (0)

0.545 (+ 1.2)

352.0 ± 29.4

26

15.00 (+ 1)

0.500 (+ 1)

302.0 ± 6.5

Model Summary

Source

DF

Adj. SS

Adj. MS

F-Value

P-Value

Coefficient

T-Value

Model

5

45,039.0

9007.8

53.99

   

Linear

2

2050.3

1025.2

6.14

0.008

  

Nitrogen Conc

1

1411.4

1411.4

8.46

0.009

12.15

2.91

Phosphate Conc

1

638.9

638.9

3.83

0.064

-8.18

-1.96

Square

2

37,570.3

18,785.2

112.59

   

Nitrogen*Nitrogen

1

9592.5

9592.5

57.50

 < 0.001

-47.13

-7.58

Phosphate* Phosphate

1

25,260.0

25,260.0

151.40

 < 0.001

-76.48

-12.30

2-Way Interaction

1

5418.4

5418.4

32.48

   

Nitrogen*Phosphate

1

5418.4

5418.4

32.48

 < 0.001

-37.48

-5.70

Error

20

3336.8

166.8

    

Lack-of-Fit

3

186.6

62.2

0.34

0.800

  

Pure Error

17

3150.2

185.3

    

Total

25

48375.8