Table 2 ANOVA, regression analysis, and significance of the Plackett–Burman experimental design to estimate the influential variables for β-glucan biosynthesis by Meyerozyma guilliermondii
Run Order | Nitrogen conc. (gl−1) | Sulfates conc. (gl−1) | Phosphates conc. (gl−1) | Minerals conc. (ml/l) | Vitamins conc. (gl−1) | β-glucan Conc. (mg/100 ml) | ||
|---|---|---|---|---|---|---|---|---|
1 | 0.7 (-1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 662.6 | ||
2 | 0.7 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.50 (+ 1) | 549.8 | ||
3 | 0.7 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 431.0 | ||
4 | 7.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 1.5 (-1) | 0.50 (+ 1) | 308.8 | ||
5 | 7.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.50 (+ 1) | 357.7 | ||
6 | 7.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 211.9 | ||
7 | 7.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 411.2 | ||
8 | 0.7 (-1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 260.7 | ||
9 | 0.7 (-1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.50 (+ 1) | 280.5 | ||
10 | 7.0 (+ 1) | 1.0 (+ 1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 299.6 | ||
11 | 0.7 (-1) | 0.1 (-1) | 0.6 (-1) | 15.0 (+ 1) | 0.05 (-1) | 212.3 | ||
12 | 0.7 (-1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.05 (-1) | 623.7 | ||
13 | 7.0 (+ 1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.05 (-1) | 523.4 | ||
14 | 0.7 (-1) | 0.1 (-1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 572.2 | ||
15 | 7.0 (+ 1) | 0.1 (-1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 442.2 | ||
16 | 0.7 (-1) | 0.1 (-1) | 6.0 (+ 1) | 15.0 (+ 1) | 0.05 (-1) | 433.6 | ||
17 | 7.0 (+ 1) | 0.1 (-1) | 6.0 (+ 1) | 1.5 (-1) | 0.50 (+ 1) | 520.1 | ||
18 | 7.0 (+ 1) | 1.0 (+ 1) | 0.6 (-1) | 15.0 (+ 1) | 0.50 (+ 1) | 190.1 | ||
19 | 7.0 (+ 1) | 0.1 (-1) | 0.6 (-1) | 1.5 (-1) | 0.05 (-1) | 312.2 | ||
20 | 0.7 (-1) | 1.0 (+ 1) | 6.0 (+ 1) | 1.5 (-1) | 0.05 (-1) | 557.7 | ||
Model Summary | ||||||||
Source | DF | Adj. SS | Adj. MS | F-Value | P-Value | Effect | Coefficient | T-Value |
Model | 5 | 145489 | 29098 | 1.57 | 0.231 | |||
Linear | 5 | 145489 | 29098 | 1.57 | 0.231 | |||
Constant | 408.1 | 13.42 | ||||||
Nitrogen | 1 | 50692 | 50692 | 2.74 | 0.120 | -100.7 | -50.3 | -1.66 |
Sulfates | 1 | 15451 | 15451 | 0.83 | 0.376 | 55.6 | 27.8 | 0.91 |
Phosphates | 1 | 66275 | 66275 | 3.58 | 0.079 | 115.1 | 57.6 | 1.89 |
Minerals | 1 | 2087 | 2087 | 0.11 | 0.742 | 20.4 | 10.2 | 0.34 |
Vitamins | 1 | 10984 | 10984 | 0.59 | 0.454 | 46.9 | 23.4 | 0.77 |
Error | 14 | 259072 | 18505 | |||||
Total | 19 | 404561 | ||||||