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Fig. 1 | BMC Microbiology

Fig. 1

From: A suite of modular, all-synthetic suicide vectors for allelic exchange mutagenesis in multidrug resistant Acinetobacter strains

Fig. 1

Map of pALFI vectors showing how they follow the SEVA architecture. The cargo segment includes the MCS (multi cloning site); AbR segment includes HygR (hygromycin resistance cassette) and AmpR (ampicillin resistance cassette); the replication segment includes R6K (pir-dependent origin of replication); an addition selection segment (which is not standard in SEVA vectors) includes SacB (levansucrase gene) and GFP (green fluorescent protein); The pALFI vectors also include an OriT (origin of conjugational transfer) and T1 (terminator). Each genetic element is flanked with unique restriction sites. The HygR resistance marker in pALFI1 is replaced with TcR in pALFI2 and with Tpm in pALFI3 (Created with BioRender.com).

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