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Table 1 Culture medium and its composition

From: Optimization of fermentation conditions for surfactin production by B. subtilis YPS-32

Medium

Composition (g/L)

NB medium

Beef extract 5, peptone 10, NaCl 5

LB medium

Yeast powder 5, peptone 10, NaCl 5

BPY medium

Beef extract 5, peptone 10, yeast powder 5, NaCl 5, glucose 10

NYD medium

Beef extract 8, yeast powder 3, glucose 1

Landy medium [19]

Glucose 20, L-glutamic acid 5, MgSO4 0.5, KCl 0.5, KH2PO4 1, FeSO4 0.00015, MnSO4 0.0005, CuSO4 0.00016

Modified Landy medium 1 (Landy 1) [20]

Glucose 20, L-glutamic acid 5, yeast powder 1, K2HPO4 1, MgSO4 -7H2 O 1.02, KCl 0.5, CuSO4 -5H2 O 0.0025, Fe2(SO4)3 0.0004, MnSO4·H2O 0.00134

Modified Landy medium 2 (Landy 2) [21]

Glucose 19.97, L-glutamic acid 13.51, yeast powder 1, K2HPO4 1, MnSO4·7H2O 0.5, KCl 0.5, L-tryptophan 0.06312, CuSO4·5H2O 0.0016, MnSO4·H2O 0.0012, Fe2(SO4)3·7H2O 0.0004