Table 1 Primers used to detect bacteria and antibiotic-resistant genes
From: Multidrug-resistant Aeromonas bacteria prevalence in Nile tilapia broodstock
Primer | Sequence | Annealing temperature (°C) | Product size (pb) | References |
|---|---|---|---|---|
Act | F: AGAAGGTGACCACCACCAAGAACA R: AACTGACATCGGCCTTGAACTC | 55 | 232 | [21] |
Ast | F: TCTCCATGCTTCCCTTCCACT R: GTGTAGGGATTGAAGAAGCCG | 55 | 331 | |
Alt | F: TGACCCAGTCCTGGCACGGC R: GGTGATCGATCACCACCAGC | 55 | 442 | |
Ser | F: CACCGAAGTATTGGGTCAGG R: GGCTCATGCGTAACTCTGGT | 50 | 350 | |
Lip | F: CAYCTGGTKCCGCTCAAG R: GTRCCGAACCAGTCGGAGAA | 58 | 247 | |
Hly | F: GGCCGGTGGCCCGAAGATACGGG R: GGCGGCGCCGGACGAGACGGGG | 55 | 592 | [48] |
SulI | F: CGGCGTGGGCTACCTGAACG R: GCCGATCGCGTGAAGTTCCG | 68 | 433 | [49] |
TetA | F: GCTACATCCTGCTTGCCTTC R: CATAGATCGCCGTGAAGAGG | 55 | 210 | [50] |
qnrs | F: ACGACATTCGTCAACTGCAA R: TAAATTGGCACCCTGTAGGC | 53 | 417 | [51] |
ermB | F: TGGTATTCCAAATGCGTAATG R: CTGTGGTATGGCGGGTAAGT | 62 | 745 | [52] |