Table 1 Characteristics of primers used for the One-Step LAMP technique [24]
Target gene | Primer sequence | Primer length (nucleotide) |
|---|---|---|
N (Nucleocapsid protein) | F3 5′-CTACCTAGGAACTGGGCC-3’ | 18 |
B3 5′-AGAAGAGGCTTGACTGCC-3’ | 18 | |
FIP 5′-GGTGTATTCAAGGCTCCCTCACCTATGGTGCTAACAAAGAC-3’ | 41 | |
BIP 5′-AATCCTGCTAACAATGCTGCAATCCTGCTCCCTTCTGCGTAG-3’ | 42 | |
LF 5′-GTTGCAACCCATATGATGC-3’ | 19 | |
LB 5′-CTTCCTCAAGGAACAACAT-3’ | 19 |