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Fig. 4 | BMC Microbiology

Fig. 4

From: Rapid, sensitive, and specific detection of SARS-CoV-2 in nasopharyngeal swab samples of suspected patients using a novel one-step loop-mediated isothermal amplification (one-step LAMP) technique

Fig. 4

Analytical and clinical sensitivity and specificity of the One-step LAMP assay for the detection of SARS-CoV-2 RNA based on the N gene amplification. A and B tenfold serial dilutions of the RNA standard of SARS-CoV-2 from 1 × 106 to 1 × 10− 3 copies per reaction. C Positive and negative SARS-CoV-2 clinical samples from participants in the study. D Various non-SARS-CoV-2 templates, including Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, Parainfluenza virus, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenza, Pseudomonas aeruginosa, Legionella pneumophila, Bordetella Pertussis, Staphylococcus aureus, Mycoplasma pneumoniae, and Chlamydia pneumoniae as well as human positive samples of HIV ، HBV ، HCV ، EBV ، CMV ، HPV, and HSV1, and 2., and synthetic nucleic acid sequences for specificity assessment of the LAMP technique. P, Positive control

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