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Table 1 Cloning primers of Pgp3 gene and primers used for qRT-PCR analysis of immune-related genes

From: Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells

Target gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

Amplicon size (bp)

Pgp3

CATG*CCATGG*GAAATTCTGGTTTTTATTTG

CGTA/CTCGAG/AGCGTTTGTTGAGGT

792

CXCL1

GCGGAAAGCTTGCCTCAA

TCAGCATCTTTTCGATGATTTTCTT

62

GM-CSF

CACTGCTGCTGAGATGAATGAAA

GTCTGTAGGCAGGTCGGCTC

78

IL-1α

TGTATGTGACTGCCCAAGATG

TTAGTGCCGTGAGTTTCCC

121

IL-5

GAGACCTTGGCACTGCTTTC

CAGTACCCCCTTGCACAGTT

157

IL-6

CCACTCACCTCTTCAGAACG

CATCTTTGGAAGGTTCAGGTTG

150

IL-8

CCTGATTTCTGCAGCTCTGT

TCTGCACCCAGTTTTCCTTG

221

TNF-α

ACTTTGGAGTGATCGGCC

GCTTGAGGGTTTGCTACAAC

139

TLR2

CAGGTGACTGCTCGGAGTTC

CACAACTACCAGTTGAAAGCAGTGA

171

  1. Restriction sites: * *, NcoI site; / /, XhoI site