Table 3 Comparison of specific and general gating strategies for the enumeration of viable bacteria
From: Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry
AFU per gram | % Difference | ||
|---|---|---|---|
Specific gating | General gating | ||
CUL21/60 | |||
Batch 1 | 3.06 × 1011 | 3.06 × 1011 | − 0.06 |
Batch 2 | 3.95 × 1011 | 3.98 × 1011 | + 0.67 |
Batch 3 | 4.96 × 1011 | 4.92 × 1011 | −0.79 |
Mean (SD) | −0.06(0.70) | ||
CUL20/34 | |||
Batch 1 | 7.14 × 1011 | 7.10 × 1011 | −0.62 |
Batch 2 | 6.43 × 1011 | 6.32 × 1011 | −1.72 |
Batch 3 | 8.57 × 1011 | 8.52 × 1011 | −0.56 |
Mean (SD) | −0.97(0.65) | ||
CUL08 | |||
Batch 1 | 6.33 × 1011 | 6.41 × 1011 | + 1.27 |
Batch 2 | 3.98 × 1011 | 4.08 × 1011 | + 2.47 |
Batch 3 | 4.01 × 1011 | 4.06 × 1011 | + 1.44 |
Mean (SD) | + 1.73(0.65) | ||
CUL66N | |||
Batch 1 | 4.84 × 1011 | 5.02 × 1011 | + 3.64 |
Batch 2 | 1.40 × 1011 | 1.47 × 1011 | + 4.90 |
Batch 3 | 4.14 × 1011 | 4.35 × 1011 | + 4.87 |
Mean (SD) | + 4.47(0.72) | ||
CUL61 | |||
Batch 1 | 2.86 × 1011 | 3.33 × 1011 | + 14.01 |
Batch 2 | 4.98 × 1011 | 5.49 × 1011 | + 9.42 |
Batch 3 | 6.90 × 1011 | 7.67 × 1011 | + 10.04 |
Mean (SD) | + 11.16(2.50) | ||