Fig. 5

Microscopic images of non-infected, and antibiotic-treated/untreated Sa30-infected C. elegans. Epifluorescence images of a Sa30 infected (24 h post-infection), b non-infected, c Sa30 infected (48 h post-infection), and d dead infected worms. Sa30 accumulation was observed in the pharynx, intestinal lumen, rectum, and anus of the worms 24 h post-infection. The increased fluorescence 48 h post-infection throughout the digestive tract of the worms suggested Sa30 multiplication leading to intestinal epithelium destruction. The antibiotics e ampicillin, f kanamycin, g streptomycin, h chloramphenicol, i tetracycline, and j ceftiofur were exposed to Sa30 infected worms for 24 h. The worms were washed and resuspended in S-basal media in a 96-well plate. Green (505 nm) and red (583 nm) filter combinations were used to acquire the green autofluorescence of C. elegans and RFP-labeled Sa30. The epifluorescence images were captured using the Cell Discoverer 7