Fig. 4

Life-span of S. aureus infected C. elegans and assessment of antibiotic efficiency. a Life-span assay of S. aureus infected and non-infected C. elegans. The worms were grown to the L4 stage and exposed to 1.5 × 10⁸ cells of S. aureus isolates for 48 h. The worms were washed, transferred to NGM plates with E. coli OP50 lawns, and monitored for survivability for 20 days. The mantel-Cox test was used to find the statistical significance (p < 0.05). b-d Antibiotic efficiency against b Sa1158c, c Sa3, and d Sa30 infection in C. elegans. The infected worms were subjected to ampicillin (AMP) (10 µg/mL), kanamycin (K) (30 µg/mL), streptomycin (S) (10 µg/mL), chloramphenicol (C) (30 µg/mL), tetracycline (TET) (30 µg/mL), and ceftiofur (CF) (30 µg/mL) for 24 h. The worms were washed and transferred to NGM plates with E. coli OP50 lawns. The survivability of the worms was monitored for 20 days. Average values plotted in the graph with different alphabets indicate a significant difference (p < 0.05). ‘NIF-Worms’ and ‘IF-Worm UT’ stands for non-infected worms and infected untreated worms, respectively. The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility