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Table 4 Primer sets and thermal profiles of transcripts for N cycle functional genes and 16S rRNA

From: Elevated atmospheric CO2 concentrations caused a shift of the metabolically active microbiome in vineyard soil

qPCR target

Primer set

Thermal cycling profile

No. cycles

Reference

16S RNA

520F, 926R complemented

95° C/45 s, 60 °C/45 s, 72 °C/60 s, 84 °C/20 s

40

[78, 79]

amoA

amoA1_F, amoA2_R

95 °C/30 s, 59 °C/30 s, 72 °C/20 s, 80 °C/20 s

35

[103]

nifH

IGK3, DVV

95 °C/20 s, 55 °C/30 s, 72 °C/30 s, 84 °C/20 s

40

[58]

nirK

nirK876, nirK 5R

95 °C/20 s, 63 °C/25 s, 72 °C/60 s, 80 °C/20 s

40

[104, 105]

nirS

Cd3aF, R3cd

95 °C/20 s, 63 °C/25 s, 72 °C/60 s, 80 °C/20 s

40

[106, 107]

nosZ

nosZ2F, nosZ2R

95 °C/30 s, 63 °C/50 s, 72 °C/50 s, 80 °C/20 s

40

[108]