Table 1 Metabolomics profile of the bioactive actinobacterial strains originated from Kubuqi desert China
Strains | Thin-layer chromatography (TLC) profile | HPLC- retention time (tR/min) | (+) and (−)-ESI-MS: (m/z) | Molecular Weight | Antimicrobial, antifungal, and cytotoxic activity | |
|---|---|---|---|---|---|---|
UV visualization | Anisaldehyde/H2SO4 Staining | |||||
PU-KB2–3 | 254 nm 3 bands 366 nm 3 bands | 3 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB2–4 | 254 nm 3 bands 366 nm 3 bands | 3 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB3–2 | 254 nm 2 bands 366 nm 2 bands | 2 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB5–11 | 254 nm 4 bands 366 nm 4 bands | 1 dark blue band, 3 light blue bands | 13.953 22.222 23.676 | 587[M + H]+, 585 [M-H]− 743[M + H]+, 777 [M + Cl]− 743[M + H]+, 777 [M + Cl]− | 586 742 742 | Highly active only against B. subtilis. Approx. 40% cytotoxic against PC3 cell line |
PU-KB5–17 | 254 nm 1 band 366 nm 4 bands | 2 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB5–19 | 254 nm 1 band 366 nm 3 bands | 3 light purple bands | 24.393 | 423[M + H]+, 421 [M-Cl]− | 422 | Highly active only against B. subtilis |
PU-KB6–2 | 254 nm 3 bands 366 nm 3 bands | 3 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB6–7 | 254 nm 1 band 366 nm 1 band | 1 orange band | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB6–10 | 254 nm 4 bands 366 nm 3 bands | 1 yellow band, 1 blue band, 1 purple band | 11.904 | 518[M + H]+, 516 [M-H]− | 517 | Highly active against only M. luteus. Approx. 40% cytotoxic against A549 and 65% against PC3 cell line |
PU-KB7–4 | 254 nm 3 bands 366 nm 6 bands | 3 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 65% cytotoxic against A549 and PC3 cell lines |
PU-KB7–7 | 254 nm 3 bands 366 nm 5 bands | 3 orange bands, 1 yellow band | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 70% cytotoxic against A549 and PC3 cell lines |
PU-KB7–8 | 254 nm 6 bands 366 nm 5 bands | 4 orange bands | 33.201 | 1255[M + H]+, 1253[M-H]− | 1254 | Approx. 70% cytotoxic against A549 and PC3 cell lines |
PU-KB8–2 | 254 nm 3 bands 366 nm 3 bands | 2 yellow bands, 1blue band | 21.387 24.852 | 1137[M + H]+, 1135[M-H]− 1119[M + H]+, 1117[M-H]− | 1136 1118 | Potent activity against B. subtilis, M. luteus and S. aureus. Approx. 70% cytotoxic against A549 and PC3 cell lines |
PU-KB9–11 | 254 nm 1 band 366 nm 1 band | 4 pale yellow bands | 15.354 | 299[M + H]+, 343[M + HCOO]− | 298 | Highly active only against B. subtilis and M. luteus. Approx. 20% cytotoxic against PC3 cell line |
PU-KB10–4 | 254 nm 2 bands 366 nm 4 bands | 0 bands | 16.934 | 460[M + H]+, 476[M-OH]− | 459 | Potent activity against B. subtilis, M. luteus and S. aureus. Highly active against Gram-negative E. coli. Approx. 40% cytotoxic against PC3 cell line |
PU-KB11–5 | 254 nm 3 bands 366 nm 3 bands | 2 pale yellow bands, 1 light purple band | 15.423 21.385 | 292[M + H]+, 290[M-H]− 1137[M + H]+, 1135[M-H]− | 291 1136 | No preliminary antimicrobial activity. Approx. 30% cytotoxic against PC3 cell line |
PU-KB12–3 | 254 nm 1 band 366 nm 0 band | 0 band | 24.078 22.982 | 373[(M-2H2O) + H]+, 407[M-H]− 466[M + H]+, 464[2 M-H]− | 408 465 | Potent activity against Bacillus subtilis, M. luteus and S. aureus |
PU-KB12–13 | 254 nm 1 band 366 nm 0 band | 0 band | 19.617 | 304[M + H]+, 302[M-H]− | 303 | Approx. 40% inhibition of S. cerevisiae |