Table 3 Comparison of recovery results of seven strains of two Astrephomene species (Table 1) after possible optimal cryogenic treatment (immature colonies of A. gubernaculifera [AG] with 6% DMF, or mature colonies of A. perforata [AP] with 3% HA; Table 2) in liquid nitrogen
From: Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene
Strain designation [species] | Total viability (range) % | Experiment I | Experiment II |
|---|---|---|---|
MPN cell numbers in three cryotubes (/mL) (control); [Number of viable cultures by 1st (2nd) inoculation with 3 (3) 10 mL cultures] | MPN cell numbers in three cryotubes (/mL) (control); [Number of viable cultures by 1st (2nd) inoculation with 3 (3) 10 mL cultures] | ||
NIES-418 [AG] | 0.570 ± 0.88 (0.0026-2.2) | 7.3, 15, 15 (280000); [3(3)] | 92, 420, 920 (42000); [3(3)] |
NIES-419a [AG] | 0 (0-0) | 0, 0, 0 (81000); [0(0)] | 0, 0, 0 (15000); [0(0)] |
NIES-628a [AG] | 0.032 ± 0.0020 (0.011-0.065) | 9.2, 11, 20 (81000); [3(3)] | 9.2, 9.2, 15 (23000); [3(2)] |
NIES-853 [AG] | 0.54 ± 0.19 (0.41-0.91) | 380, 420, 740 (81000); [3(3)] | 38, 42, 42 (9200); [3(3)] |
NIES-854a [AG] | 0 (0-0) | 0, 0, 0 (40000); [0(0)] | 0, 0, 0 (9200); [1(0)] |
NIES-855a [AG] | 0 (0-0) | 0, 0, 0 (180000); [0(0)] | 0, 0, 0 (23000); [0(0)] |
NIES-565 [AP] | 9.6 ± 5.0 (3.8-18) | 1500, 2300, 4200 (40000); 3 [3] | 2100, 2300, 4200 (23000); [3(3)] |