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Table 2 Statistics summary for the 17 Chloroflexi MAGs recovered in this study

From: Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors

Reactor

Genome

Genome size (bp

CDSa

Completenessb/ Contamination

Contigs

N50 (bp)

16S/23S/5Sd

tRNAa

%GC

Coverage

Taxonomic annotationc

Closest placement ANI %

Activated sludge

RH21

5,657,355

5097

85.59/0

655

12,275

1(922nt)/1(492nt)/1

4 5

56.34

8.2

c_Anaerolineae;o_SBR1031;f_A4b;g_OLB15

76.07

RH38

5,163,972

4600

87.29/1.73

1167

5326

1(805nt)/1(342nt)/2

32

64.57

8.6

c_Anaerolineae;o_Caldilineales;f_Caldilineaceae

N/A

RH43

9,980,378

8014

90.86/4.7

129

94,438

1(807nt)/1/1

44

55.88

3.03

c_Anaerolineae;o_Caldilineales;f_Caldilineaceae;g_CFX5

76.3

RH52

4,922,116

3510

96.79/1.1

201

35,320

1(62nt)/1/1

57

73.22

20.4

c_Anaerolineae;o_UCB3;f_UCB3;g_UCB3;s_UCB3 sp003576755

99.2

Methanogenic

MO16

3,073,917

2871

86.36/3.36

387

11,990

1(1118nt)/0/0

35

58.98

10

c_Anaerolineae;o_Anaerolineales;f_Anaerolineaceae;g_Longilinea

79.35

MO53

4,940,107

4005

87.27/0.91

227

44,487

1(337nt)/1(370nt)/1

45

58.31

13.5

c_Anaerolineae;o_B4-G1;f_SLSP01

N/A

MO66

4,384,884

3803

92.73/3.36

478

14,973

0/0/0

44

64.91

14.7

c_Anaerolineae;o_UBA3071;f_CG2-30–64-16

N/A

MO118

3,352,740

2734

90.18/2.73

86

51,993

1(847nt)/1(337nt)/1

38

41.05

13.2

c_Anaerolineae;o_Anaerolineales;f_Anaerolineaceae;g_Flexilinea

83.18

Anammox

AMX9

4,128,696

3450

98.18/0

54

178,058

1(800nt)/1(617nt)/1

48

62.69

32.7

c_Anaerolineae;o_SBR1031;f_A4b;g_OLB13;s_OLB13 sp001567485

99.66

AMX14

3,390,571

3072

92.73/0.91

124

43,811

1(329nt)/0/0

41

56.87

25.1

c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_OLB14;s_OLB14 sp900696595

99.72

AMX15

3,646,596

3399

90.91/0.18

38

293,047

1(534nt)/2/1

42

54.08

160.3

c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA12294;s_UBA12294 sp003577395

95.27

AMX39

2,675,910

2460

90.91/0.91

18

265,768

1(445nt)/0/0

48

52.31

19.8

c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA12294;s_UBA12294 sp002050275

99.2

AMX47

2,814,255

2926

91.21/0

319

11,859

1(1498nt, 1518nt)/1/1

42

66.7

10.4

c_Dehalococcoidia;o_UBA2991;f_UBA2991;g_UCB2

82.41

AMX55

2,990,515

2772

92.73/1.27

61

69,781

1(1509nt)/1(317nt)/1

40

60.81

15.1

c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_UBA7227;s_UBA7227 sp002473085

98.72

AMX56

4,499,265

3993

92.42/1.09

96

86,147

3(1218–1493)/2/2

58

63.48

54.1

c_Anaerolineae;o_Caldilineales;f_J102

76.67

AMX57

4,700,319

4113

92.73/0

236

29,630

1(703nt)/1(461nt)/1

46

60.87

13.6

c_Anaerolineae;o_SBR1031;f_A4b;g_GCA-2702065

75.87

AMX68

4,329,913

4110

92.73/0.91

70

171,406

1(195nt)/1(219nt)/1

46

53.34

15.8

c_Anaerolineae;o_Anaerolineales;f_EnvOPS12;g_OLB14

79.8

  1. N/A Not assigned
  2. aAs predicted using Prokka (details in Methods section)
  3. bGenome quality estimates from CheckM (details in Methods section)
  4. cTaxonomic assignments from GTDB-Tk (details in Methods section)
  5. dSequences obtained from Barrnap (details in Methods section)
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BMC Microbiology

ISSN: 1471-2180

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