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Fig. 3 | BMC Microbiology

Fig. 3

From: Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors

Fig. 3

Phylogenomic and phylogenetic analysis of Chloroflexi based on the metagenomics results and the 16S rRNA gene sequence analysis: A) Phylogenomic tree constructed with the 17 genomes from this work and reference genomes retrieved from the NCBI, B) Phylogenetic tree constructed with the most abundant ASVs retrieved from the 16S rRNA gene amplicon sequences (with abundance higher than 5% in at least one sample) and the sequences of the 16S rRNA gene from 10 MAGs from this work, and reference sequences of the phylum Chloroflexi retrieved from NCBI. The tree was reconstructed using the ML method and the GTR model. ML bootstrap values greater than or equal to 70% are shown at each node. Bar indicates 0.01 substitutions per site. The ASV and MAGs are colored by reactor: blue for RH, orange for MO and green for AMX. Sequences from the phylum Thermotogota were used as outgroup for rooting trees

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