Table 4 Primers used for MLST of A. baumannii complex (Oxford scheme)
Locus | Primer | Sequences | Amplicon size (bp) | Usage |
|---|---|---|---|---|
gltA | Citrato F1 | AAT TTA CAG TGG CAC ATT AGG TCC C | 722 | amp/seq |
Citrato R12 | GCA GAG ATA CCA GCA GAG ATA CAC G | amp/seq | ||
gyrB | gyrB_F | TGA AGG CGG CTT ATC TGA GT | 594 | amp/seq |
gyrB_R | GCT GGG TCT TTT TCC TGA CA | amp/seq | ||
gdhB | GDHB 1F | GCT ACT TTT ATG CAA CAG AGC C | 774 | amp |
GDH SEC F | ACC ACA TGC TTT GTT ATG | seq | ||
GDHB 775R | GTT GAG TTG GCG TAT GTT GTG C | amp | ||
GDH SEC R | GTT GGC GTA TGT TGT GC | seq | ||
recA | RA1 | CCT GAA TCT TCY GGT AAA AC | 425 | amp/seq |
RA2 | GTT TCT GGG CTG CCA AAC ATT AC | amp/seq | ||
cpn60 | cpn60_F | GGT GCT CAA CTT GTT CGT GA | 640 | amp/seq |
cpn60_R | CAC CGA AAC CAG GAG CTT TA | amp/seq | ||
gpi | gpi_F | GAA ATT TCC GGA GCT CAC AA | 456 | amp/seq |
gpi_R | TCA GGA GCA ATA CCC CAC TC | amp/seq | ||
rpoD | rpoD-F | ACC CGT GAA GGT GAA ATC AG | 672 | amp/seq |
rpoD-R | TTC AGC TGG AGC TTT AGC AAT | amp/seq |