Skip to main content

Table 1 Overall purification profile of cytosine deaminase from A. niger

From: Aspergillus Niger thermostable Cytosine deaminase-dextran conjugates with enhanced structure stability, proteolytic resistance, and Antiproliferative activity

Step

Volume (ml)

Total activity (U)

Total protein (mg)

Specific activity (μmol/mg/min)

Recovery (%)

Purification fold

Crude Enzyme

37

28,672.68

524.82

54.63

100.00

1.00

Dialysis

4

2194.84

20.20

108.68

7.65

1.99

Sephadex G100

9

7107.84

31.08

228.70

24.79

4.19

DEAE-Sepharose

10

11,475.36

13.58

844.93

40.02

15.47