Table 2 The purification efficiency of the extracellular protease from strain NJSZ-13
Steps | Total protein(mg) | Total enzyme activity(U) | Specific activity(U/mg) | Purification efficiency |
|---|---|---|---|---|
Culture filtrate | 41.5 | 1959.6 | 47.2 | 1.0 |
Salting out of ammonium sulfate | 16.1 | 1732.8 | 107.6 | 2.3 |
HIC | 7.7 | 1514.3 | 196.7 | 4.2 |
IEC | 4.3 | 1258.2 | 292.6 | 6.2 |