Fig. 3
From: A Zur-mediated transcriptional regulation of the zinc export system in Pseudomonas aeruginosa
Mutations in the Zur boxes and their effects on Zur binding activity. A) Sequences of modified Zur boxes: mut1, mut2 and mut1 + 2 in red. B) Electrophoretic Mobility Shift Assay (EMSA) using purified Zur protein, different czcR 5’-UTR DNA region (czcR) and znuB (negative DNA control). 25 ng of each DNA per reaction was used. The Zur protein and Zn concentrations are indicated at the top of the figure. czcR wt, corresponds to the wt sequence of the czcR 5’-UTR DNA region, czcR mut1 and mut2 possess mutated box 1 or 2, respectively; czcR mut1 + 2 possess the two mutated Zur boxes