Fig. 1

Zur binds upstream the czcR translation start codon. A) Electrophoretic Mobility Shift Assay (EMSA) using purified Zur protein and czcR-5’UTR DNA region or znuB ORF DNA region as negative control. 25 ng of DNA per reaction was used. The Zur protein, Zn and TPEN (Zn chelator) concentrations are indicated at the top of the figure. B) DNAse I footprinting of czcR promoter in the absence or presence or of 1 µM Zur protein. DNA fragments were analyzed by capillary electrophoresis. The two Zur boxes are indicated by numbers 1 and 2 in red. The sequencing reaction is visible below the figure (seq2). C) Sequence of the two boxes as determined by the footprinting experiments (Fig. 1B coding strand; Fig S1A template strand)