From: Optimization of glutathione production in Saccharomyces cerevisiae HBSD-W08 using Plackett–Burman and central composite rotatable designs
Number
Factor
Level
− 1
0
+ 1
A
Glucose
3%
4%
5%
B
Peptone
C
KH2PO4
0.25
0.5
0.75
D
Magnesium Sulfate
0.5%
1%
1.5%
E
Ammonium Sulfate
0.75%
1.0%
F
Glutamate
0.1%
0.15%
0.2%