Table 5 Primer sequences used for RT-qPCR
Gene symbol | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Accession number |
|---|---|---|---|
GAPDH | AAGGTGAAGGTCGGAGTCAA | ATGACAAGCTTCCCGTTCTC | NM_002046 |
EREG | CTTATCACAGTCGTCGGTTCCAC | GCCATTCAGACTTGCGGCAACT | NM_001432 |
FOXF1 | AGCAGCCGTATCTGCACCAGAA | CTCCTTTCGGTCACACATGCTG | NM_001451 |
PTGS2 | CGGTGAAACTCTGGCTAGACAG | GCAAACCGTAGATGCTCAGGGA | NM_000963 |
SPP1 | CGAGGTGATAGTGTGGTTTATGG | GCACCATTCAACTCCTCGCTTTC | NM_000582 |
TNFRSF1B | CGTTCTCCAACACGACTTCATCC | ACGTGCAGACTGCATCCATGCT | NM_001066 |
CLDN18 | ATGGAGGACTCTGCCAAAGCCA | TGGACATCCAGAAGTTAGTCACC | NM_016369 |
ALDH1A3 | CTGCTACAACGCCCTCTATGCA | GTCGCCAAGTTTGATGGTGACAG | NM_000693 |