Fig. 3From: Carotenoid biosynthesis is associated with low-temperature adaptation in Rhodosporidium kratochvilovaeThe schematic diagram of the Cas9-expression cassette. The synthesized codon-optimized 6*His-NLS-fused Cas9 was inserted the Nco I and EcoR V sites of the vector pRH034 under the control of the GPD promoter (pGPD1)Back to article page