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Table 1 The prediction ability of IAV-infectivity using LR-RT-PCR targeting the PA segment

From: Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses

Treatment

Evaluation ability

Needed information

UV irradiation [18]

Copy number after treatment only

Heating

Copy number after treatment only

*Over 5 min treatment and use of the cutoff value (= LoD) are required

Sodium hypochlorite

Copy numbers both before and after treatment

*Copy number decrease: ~ 1 digit

Ethanol

Cannot predict

  1. The table indicates the ability of LR-RT-qPCR in assessing the infectivity of IAV and needed information for the evaluation. The evaluation ability is conveniently indicated by symbols
  2. : The LR-RT-qPCR can detect infectious IAV to distinguish it from IAV inactivated by the treatment
  3. : The LR-RT-qPCR can be applied to confirm the effect of the sanitization
  4. ✕: The LR-RT-qPCR cannot evaluate infectivity of IAV inactivated by the treatment