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Fig. 2 | BMC Microbiology

Fig. 2

From: Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses

Fig. 2

Effect of 0.12% sodium hypochlorite on IAV (Panama strain) titers and copy numbers. IAV suspensions were treated with 0.12% sodium hypochlorite for increasing periods between 10 and 30 min. The titer and copy numbers of treated IAV were determined by TCID50 assay using MDCK cells and by LR-RT-qPCR targeting the PA segment, respectively. The titers are plotted on the graph and shown as log (TCID50/0.1 mL) (). Assays were carried out independently three times with quadruplicate wells per dilution and values represent the mean ± standard error. After over 10 min treatment, there was no CPE in all conditions. They were defined as 0 (means ‘Not detected’) for convenience. The ratio of copy numbers obtained using LR-RT-qPCR is also plotted on the graph (). Assays were performed with triplicate wells per sample and plots represent the mean ± standard error and individual values. A broken line indicated the LoD of LR-RT-qPCR. The data indicated in Additional Table 2 (See ‘Additional Tables’)

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