Table 1 Bacterial strains and plasmids

 DSM 20016

Type strain, plasmid free; Cm^s, Em^s, Tet^s

DSM^a; Kandler et al. (1980) [3]

 TG1

Recipient in transformation; F′ traD36

New England Biolabs;

lac I^q,Δ (lacZ)M15 proA⁺B⁺/supE

Maniatis et al. (1989) [27]

Δ (hsdM-mcrB)5 (r_k⁻m_k⁻McrB⁻) thi

Δ (lac-proAB), Em^s, Ap^s

 pUC19

E.coli cloning vectors; 2.7-kb, Ap^r, lacZ

Yanisch-Perron et al. (1985) [28]

 pTE15/19

pUC19 plus the 15-kb XbaI DNA

This study

fragment from pTE15; 17.7-kb, Ap^r, Em^r

 pUE80 (−)

pUC19 plus the 1.0-kb HindIII-HindIII

Lin C-F. et al. (1999) [18]

DNA fragment from pUE8010; 3.8-kb; Ap^r, Em^r

 pTE15-RR

pUE80 (−) plus the 6.8-kb BsrBI-BsrBI

This study

DNA fragment from pTE15/19; 10.6-kb; Ap^r, Em^r

 pTE15-RO

Deletion derivative of pTE15RR; containing a 3.1-kb

Lin and Chung (1999) [18]

BsrBI-PstI fragment of pTE15

 pTE15-RRΔ1

Deletion derivative of pTE15-RO; containing a 2.9-kb

This study

BsrBI-BglII fragment of pTE15

 pTE15-RRΔ2

Deletion derivative of pTE15-RO; containing a 2.6-kb

This study

BsrBI-EcoRV fragment of pTE15

 pTE15-RRΔ3

Deletion derivative of pTE15-RO; containing a 2.1-kb

This study

BsrBI-BamHI fragment of pTE15

 pTE15-RRΔ4

Deletion derivative of pTE15-RO; containing a 2.3-kb

This study

HindIII-PstI fragment of pTE15

 pTE15-RRΔ5

Deletion derivative of pTE15-RO; containing a 2.1-kb

This study

SacI-PstI fragment of pTE15

 pTE15-RRΔ6

Deletion derivative of pTE15-RO; deleting a 0.98-kb

This study

SacI-PvuI fragment of pTE15-RO

 pTE15-RRΔ7

Deletion derivative of pTE15-RO; containing a 1.3-kb

This study

HindIII-BamHI fragment of pTE15