Fig. 4

Functional analysis of the replication region of the L. reuteri plasmid pTE15. A linear restriction map is shown at the bottom of the diagram. Putative ORFs (black arrows), cis-acting region (light grey box), and promoters (arrowheads) shown on the restriction map were inferred from sequence analysis. Think lines above the linear map represent the fragments cloned into pUE80 (−). The names of recombinant plasmids containing these inserts are indicated on the left. The replication phenotype (Rep) is indicated on the right. The vertical lines delimit the core replicon