Fig. 3

DNA sequence of the 3487-bp fragment of pTE15 containing all the information required for autonomous replication. A Schematic representation of the pTE15 minireplicon contained IR, SDR, LDR (AT-rich, iteron), DnaA box, and cer-like site. B The putative amino acid sequences of RepA, RepB and RepC are presented below the DNA sequence. Possible − 35, − 10, and ribosome binding sites (RBS) are presented upper the lowercase DNA sequence; inverted repeated (IR) sequences are indicated with single-line arrows; possible iteron DNA sequences (TAGTRRR) of pAD1 are presented italic word. The following characteristics are indicated: =DnaA is a DnaA-like box; cer like site is a cer-like resolution site. Regions characterized by a high AT content and including direct repeats are bolded and shaded, except the CTTGCTCTCTC sequence. The following short ORFs, lacking a potential RBS at an appropriate distance from the putative start codon, are present upstream of the transcribed repB and repC: (i) lower strand, position 1029–901, MLMGQGQGAF-KLSALQLLMS-SAAVFAKLIN-DCDGLTERAM-FS*; (ii) lower strand, position 1127–1023, MMVSHSSNSL-ICCLLNKKNQ-PAEQGLGGNT-RACE*; (iii) upper strand, position 1149–1238, MGYLKHFQGF-LATLLVLCDK-HWDWNSIDC*; (iv) upper strand, position 1281–1442, MRLRCRATAS-EFTGCDPKEK-ACSRLATHFL-SSSGFNSSFD-NLNFSPFLSI-LYI*