Skip to main content
Fig. 2 | BMC Microbiology

Fig. 2

From: Characterization of a novel theta-type replicon of indigenous plasmid pTE15 from Lactobacillus reuteri N16

Fig. 2

Identification of the replication region of pTE15 by DNA electrophoresis. The recombinant plasmids were undigested by restriction enzyme; lane M1: supercoiled DNA marker (Sigma-Aldrich), lanes 1, 2: pUE80 (−) (3.8-kb) and pTE15-RO (6.9-kb) were purified from E. coli TG1, lane 3: pTE15-RO was purified from L. reuteri 20016. The recombinant plasmids were digested by XbaI/PstI, lanes 4, 5: pUE80 (−) and pTE15-RO were purified from E. coli TG1, lane 6: pTE15-RO was purified from recombinant L. reuteri DSM 20016. lane M2: λDNA/HindIII marker

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us