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Fig. 8 | BMC Microbiology

Fig. 8

From: Evaluation of the antibacterial, antibiofilm, and anti-virulence effects of acetic acid and the related mechanisms on colistin-resistant Pseudomonas aeruginosa

Fig. 8

The potential antibacterial mechanism of AA. A, B Effects of AA and HA corresponding to the pH of each concentration of AA on the outer membrane permeability of TL1671 and TL2314, respectively; C, D Effects of AA and HA corresponding to the pH of each concentration of AA on the inner membrane permeability of TL1671 and TL2314 by using a plate reader, respectively; E Effect of AA on the inner membrane permeability of TL1671 and TL2314 by a fluorescence microscope. a TL1671 treated with the LB broth control; b–e TL1671 treated with 1/16 × MIC, 1/8 × MIC, 1/4 × MIC, 1/2 × MIC AA, respectively; f TL2314 treated with the LB broth control; g–j TL1671 exposed to 1/16 × MIC, 1/8 × MIC, 1/4 × MIC, 1/2 × MIC AA, respectively. Data were analyzed by Student’s t-test; ns, not statistically significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. The experiments were performed thrice. The data were expressed as the mean ± standard deviation

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