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Table 3 List of oligonucleotide sequences (primers) and cycling conditions used in the study

From: Prevalence and molecular characterization of Mycobacterium tuberculosis complex in cattle and humans, Maiduguri, Borno state, Nigeria: a cross-sectional study

Primer name

Primer sequence (oligonucleotide sequences)

Product size

enzyme activation

Cycles [30]

Incubation

Denaturation

Annealing

Extension

DRa

GGTTTTGGGTCTGACGAC, 59 biotinylated

 

96 °C

15 min.

96 °C

1 min

55 °C

1 min

72 °C

30 sec.

42 °C

45–60 min.

DRb

CCGAGAGGGGACGGAAAC

 

96 °C

15 min.

96 °C

1 min

55 °C

1 min

72 °C

30 sec.

42 °C

45–60 min.

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BMC Microbiology

ISSN: 1471-2180

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