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Fig. 2 | BMC Microbiology

Fig. 2

From: Mutation of gdpS gene induces a viable but non-culturable state in Staphylococcus epidermidis and changes in the global transcriptional profile

Fig. 2

Mutation of gdpS induces S. epidermidis to enter into a VBNC state under osmotic pressure. All the cryopreserved S. epidermidis strains were resuscitated on Columbia blood agar plate and single colonies were inoculated into liquid TSB medium for overnight culture. The overnight cultures were subcultured 1:100 and grown to the exponential phase with identical OD600 values in fresh TSB medium, and then serially diluted (1:10), spotted onto TSB agar plate (A) and Columbia blood agar plate (B) for cultivation, respectively. The experiment was repeated at least three times, and a representative figure is shown

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BMC Microbiology

ISSN: 1471-2180

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