Fig. 4

Gut microbiota dysbiosis and serum total bile acid may be related to hepatic fibrosis severity. Göttingen Minipigs were fed either a control (n = 5) or choline-deficient amino acid defined high fat diet (CDAHFD) (n = 7) for 8 weeks starting at age 8 weeks. A Cholesterol level (mmol/L) (previously published by Pedersen and colleagues (2020) [23]) and B Serum total bile acid (µmol/L); 16S rRNA amplicon sequencing was used to determine the composition of the colon microbiome and was found to contain C Ruminococcus genus (Relative abundance (%)) D Hepatic fibrosis level determined using histology pico Sirius Red image analysis (µm²/cell) (previously published by Pedersen and colleagues (2020) [24]). Data are shown as individual values with mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 mark the level of statistical significance