Fig. 1
CDAHFD fed Göttingen Minipigs experienced a decreased amount of short-chain fatty acid resulting in the colon increasing the pH Colon microbiome composition of Göttingen Minipigs after 8 weeks of either control (n = 5) or choline-deficient amino acid defined high fat diet (CDAHFD) (n = 7) starting at age 8 weeks as determined by 16S rRNA amplicon gene sequencing: A Taxa summary - Bar plot illustrating the 28 most abundant bacterial families present as a consequence of differential feeing B PCoA plot using GUniFrac distance showing clear separation of the colon microbiota as a consequence of differential feeding; PERMANOVA, ANOSIM: R2 = 0.475, p = 0.0010, and C Boxplot showing Alpha diversity (Chao1 index) (25th percentile, 75th percentile, median, whiskers indicate max and min value; student’s t-test: p = 0.0000014); D Relative abundance (%) of bacterial families Lachnospiraceae and Enterobacteriaceae; E Metabolomics was used to determine the three most abundant short chain fatty acid (SCFA) butyric-, propionate- and acetic-acids (µg/g colon content) in the colon microbiota (mean + SEM); F pH measurement of colon content. Data are shown as individual values with mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 mark the level of statistical significance