Fig. 2From: Association between gut microbiota and longevity: a genetic correlation and mendelian randomization study Basic principles of Mendelian randomization. Mendelian randomization can be used to test the hypothesis that gut microbiota causes longevity, provided that three key assumptions are met adequately: (1) the IVs are robustly associated with exposure; (2) the IVs are not associated with confounders; (3) the IVs have no association with the outcome except through the exposureBack to article page