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Fig. 5 | BMC Microbiology

Fig. 5

From: Anti-Helicobacter pylori activity of potential probiotic Lactiplantibacillus pentosus SLC13

Fig. 5

Anti-H. pylori-induced inflammation of lactobacilli and their derived exopolysaccharide. GES-1 (A) and AGS (B) cells were pretreated with lactobacilli SLC13, LGG, or BCRC 14619 T for 2 h, and then cells were infected with H. pylori J99 (MOI = 100) for 2 h. C. AGS cells were treated with EPS extracted from lactobacilli SLC13, LGG, or BCRC 14619 T at a concentration of 500 ng/mL with H. pylori J99 (MOI = 100) simultaneously for 2 h. After H. pylori infection, RNA was extracted from cells to determine the il-8 gene expression. AGS and GES-1 cells alone without any treatments were used as a negative control to normalize the measurements. Error bars represent the standard deviation of biological triplicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, no significant difference; EPS, exopolysaccharide

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