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Fig. 2 | BMC Microbiology

Fig. 2

From: Anti-Helicobacter pylori activity of potential probiotic Lactiplantibacillus pentosus SLC13

Fig. 2

Anti-H. pylori activity of lactobacilli. A The inhibitory effect of lactobacilli on H. pylori J99 was determined by disk diffusion method. MRS broth alone was used as a negative control. B The survival rate of H. pylori in the presence of different concentrations (10%, 20%, and 40%) of CFS or neutralized-CFS (adjusted to pH 6.5 by NaOH) at microaerophilic conditions for 4 h. The viability of H. pylori after 72 h co-incubation with CFS was evaluated by determining the viable bacterial count on Brucella agar containing 10% horse serum plates after incubation at 37 °C under microaerophilic conditions. MRS broth pH 4.0 (acid-CFS control) and pH 6.5 (neutralized-CFS control) were used as controls of the reaction. CFS, cell-free supernatant. Error bars represent the standard deviation of biological triplicates. *, p < 0.05; **, p < 0.01; ns, no significant difference

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