Fig. 1
Flowchart of the reconstruction of SigB promoter binding motif for Bacillus subtilis. All SigB PBMs were predicted using the MEME Suite version 5.0.5 [35, 36], and MAST was used to screen the genome for a potential SigB PBM, as described by Wels et al. [32, 33]. The letter N indicates the number of base pairs present in the spacer region. STD stands for the standard motif, which was built based on all the listed SigB regulon genes on Subtiwiki up to October 2020 [13]. The p-value cut-off threshold, indicating the confidence level of the predicted motif, was set as 105. All final sequences predicted for B. subtilis 168 were manually curated and listed in Supplementary Table S2