Fig. 8

In vivo survival test of S. aureus. 6 groups of healthy mice comprising 10 mice each were used. Two negative control groups either injected with sterile PBS or kept uninfected and a positive control group injected with untreated S. aureus. 3 test groups were injected with ascorbic acid, dexamethasone, and sodium bicarbonate-treated S. aureus. Mice survival in each group was observed every day for 3 days, plotted using the Kaplan-Meier method, GraphPad Prism 8. All mice in the negative control groups survived, while only 40% of mice survived in the positive control group. Ascorbic acid in sub-MIC (12.5 mg/ml) protected all mice, conferring 60% increased protection in comparison with the group inoculated with untreated bacteria. Meanwhile, dexamethasone, and sodium bicarbonate in sub-MIC (2 mg and 4 mg/ml); (6 mice survived) confer 20% increased protection in comparison with the group inoculated with untreated bacteria