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Fig. 6 | BMC Microbiology

Fig. 6

From: The electronic tree of life (eToL): a net of long probes to characterize the microbiome from RNA-seq data

Fig. 6

Overlaps within the probe collection ('redundancy'). (A) The collection was compared to itself, revealing that over 60% were unique. (B) To determine how probe overlaps might affect sequence detection, we compared the total readcounts (brain) to the extent of probe redundancy (number of overlaps in the probe collection), demonstrating that high readcounts do not correlate with redundancy. The r2 of the trendline (Microsoft Excel) was 0.02, showing that only 2% of the observed variation in numbers of matches can be ascribed to probe redundancy. (C,D) Side-by-side comparison of probe redundancy (C) with brain readcounts (D), demonstrating no obvious correlation. (E) As in (D), but the readcount scores have been divided by the redundancy of each probe, showing some changes, but conservation of the overall pattern. (F) Effect of removing probe signals with different levels of redundancy (≥20, ≥10, ≥5, and >1). Although the overall profile was retained, this degraded many signals, indicating that the complete probe list is preferable for comprehensive retrieval

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