Fig. 3From: Surface layer protein A from hypervirulent Clostridioides difficile ribotypes induce significant changes in the gene expression of tight junctions and inflammatory response in human intestinal epithelial cellsRelative mRNA levels and cytokine measurements. Relative expression of (A) TLR-4 (B) IL-8 (C) TNF-α (D) IL-1β, (E) IL-6 genes measured by using quantitative real-time PCR assay and inflammatory cytokines production of (F) IL-8, (G) TNF-α (H) IL-1β measured by ELISA in HT-29 cells upon treatment with SlpA (20 µg/mL) from C. difficile (RT126, RT001, RT084) and C. difficile ATCC 700057 at different time points (4, 8, 12 and 24 h). All Data were normalized to β-actin as the reference. Data were presented as mean ± SD from three independent experiments. Adjusted P-values (padj) less than 0.05 were considered statistically significant; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Bonferroni method and ANOVA statistical analysis. RT, ribotypeBack to article page