Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: Surface layer protein A from hypervirulent Clostridioides difficile ribotypes induce significant changes in the gene expression of tight junctions and inflammatory response in human intestinal epithelial cells

Fig. 3

Relative mRNA levels and cytokine measurements. Relative expression of (A) TLR-4 (B) IL-8 (C) TNF-α (D) IL-1β, (E) IL-6 genes measured by using quantitative real-time PCR assay and inflammatory cytokines production of (F) IL-8, (G) TNF-α (H) IL-1β measured by ELISA in HT-29 cells upon treatment with SlpA (20 µg/mL) from C. difficile (RT126, RT001, RT084) and C. difficile ATCC 700057 at different time points (4, 8, 12 and 24 h). All Data were normalized to β-actin as the reference. Data were presented as mean ± SD from three independent experiments. Adjusted P-values (padj) less than 0.05 were considered statistically significant; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Bonferroni method and ANOVA statistical analysis. RT, ribotype

Back to article page