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Fig. 2 | BMC Microbiology

Fig. 2

From: Surface layer protein A from hypervirulent Clostridioides difficile ribotypes induce significant changes in the gene expression of tight junctions and inflammatory response in human intestinal epithelial cells

Fig. 2

Relative mRNA levels of intestinal tight junction genes in HT-29 cells upon treatment with SlpA (20 µg/mL) from C. difficile (RT126, RT001, RT084) and C. difficile ATCC 700057 at different time points (4, 8, 12 and 24 h) measured by using quantitative real-time PCR assay. (A) Claudin-1, (B) Claudin-3, (C) Claudin-7, (D) JAM-A, (E) Occludin, (F) E-cadherin, (G) ZO-1, (H) ZO-2. Gene expression data were normalized to β-actin as the reference gene. Data were presented as mean ± SD from three independent experiments. Adjusted P-values (padj) less than 0.05 were considered statistically significant; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Bonferroni method and ANOVA statistical analysis. RT, ribotype

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BMC Microbiology

ISSN: 1471-2180

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