Fig. 1From: Surface layer protein A from hypervirulent Clostridioides difficile ribotypes induce significant changes in the gene expression of tight junctions and inflammatory response in human intestinal epithelial cellsCell viability determined by MTT assay. Different concentrations (15, 20, 25 µg/mL) of SlpA from (A) RT126 (B) RT001 (C) RT084 and (D) ATCC 700057 of C. difficile strains were added to HT-29 cells for different time points (4, 8, 12, 24 and 48 h) at 37 °C. Data were presented as mean ± SD from three independent experiments. Adjusted P-values (padj) less than 0.05 were considered statistically significant; *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by Bonferroni method and ANOVA statistical analysis. RT, ribotypeBack to article page