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Table 3 MIC and MBC values of biocides in presence and absence of EDTA17% determined for 97 S. maltophilia isolates

From: Evaluation of antibacterial activity of five biocides and the synergistic effect of biocide/EDTA combinations on biofilm-producing and non-producing Stenotrophomonas maltophilia strains isolated from clinical specimens in Iran

 

Dilution rang tested N (%)

Biocides

Serial dilution

1/2

1/4

1/8

1/16

1/32

1/64

1/128

1/256

1/512

Sodium hypochlorite 5%

Active ingredients

2.5%

1.2%

0.62%

0.31%

0.15%

0.078%

0.039%

0.019%

0.0097%

MIC

–

–

–

–

–

24 (24.7%)

43 (44.3%)

27 (27.8%)

3 (3.1%)

MIC+EDTA

–

–

–

–

4 (4.1%)

4 (4.1%)

4 (4.1%)

27 (27.8%)

58 (59.8%)

MBC

–

–

–

–

–

45 (46.4%)

36 (37.1%)

15 (15.5%)

1 (1%)

MBC + EDTA

–

–

–

–

5 (5.2%)

6 (6.2%)

15 (15.5%)

64 (66%)

7 (7.2%)

Dettol 4.8%

Active ingredients

2.4%

1.2%

0.62%

0.31%

0.15%

0.075%

0.037%

0.018%

0.0093%

MIC

–

–

–

–

–

32 (33.0%)

50 (51.5%)

15 (15.5%)

–

MIC+EDTA

–

–

–

–

–

8 (8.2%)

10 (10.3%)

48 (49.5%)

31(32%)

MBC

–

–

–

–

11 (11.3%)

56 (57.7%)

28 (28.9%)

2 (2.1%)

–

MBC + EDTA

–

–

–

–

3 (3.1%)

8 (8.2%)

27 (27.8%)

57 (58.8%)

2 (2.1%)

Chlorhexidine 2%

Active ingredients

1%

0.5%

0.25%

0.125%

0.062%

0.031%

0.015%

0.0078%

0.0039%

MIC

–

–

–

–

17 (17.5%)

42 (43.3%)

37 (38.1%)

1 (1%)

–

MIC+EDTA

–

–

–

2 (2.1%)

6 (6.2%)

1 (1%)

23 (23.7%)

62 (63.9%)

3 (3.1%)

MBC

–

–

–

–

44 (45.4%)

41 (42.3%)

11 (11.3%)

1 (1%)

–

MBC + EDTA

–

–

–

2 (2.1%)

6 (6.2%)

16 (16.5%)

45 (46.4%)

28 (28.9%)

–

Sayasept-HP 2%

Active ingredients

1%

0.5%

0.25%

0.125%

0.062%

0.031%

0.015%

0.0078%

0.0039%

MIC

–

–

–

2 (2.1%)

38 (39.2%)

41 (42.3%)

16 (16.5%)

–

–

MIC+EDTA

–

–

–

3 (3.1%)

5 (5.2%)

11 (11.3%)

41 (42.3%)

37 (38.1%)

–

MBC

–

–

–

21 (21.6%)

49 (50.5%)

26 (26.8%)

1 (1%)

–

–

MBC + EDTA

–

–

–

8 (8.2%)

3 (3.1%)

33 (34%)

50 (51.5%)

3 (3.1%)

–

Ethyl Alcohol 70%

Active ingredients

35%

17.5%

8.78%

4.37%

2.18%

1.09%

0.54%

0.27%

0.13%

MIC

–

–

2 (2.1%)

38 (39.2%)

41 (42.3%)

15 (15.5%)

1 (1%)

–

–

MIC+EDTA

–

–

–

7 (7.2%)

3 (3.1%)

32 (33%)

46 (47.4%)

9 (9.3%)

–

MBC

–

–

35 (36.1%)

32 (33%)

30 (30.9%)

–

–

–

–

MBC + EDTA

–

–

5 (5.2%)

6 (6.2%)

33 (34%)

38 (39.2%)

15 (15.5%)

–

–

  1. The cells (−) means is not MIC and MBC for any of isolates
  2. Two wells were growth (TSB + inoculation) and sterility (contained TSB alone) controls