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Table 4 Repeatability of the LAMP on chip

From: Rapid and simultaneous detection of multiple pathogens in the lower reproductive tract during pregnancy based on loop-mediated isothermal amplification-microfluidic chip

Concentration

No

PQC*

GBS

EF

GV

CA

CT

Medium positive

1

15.5

11.0

13.0

17.0

15.0

8.0

2

16.5

10.5

12.0

16.0

15.5

8.0

3

15.5

11.5

13.5

15.5

15.5

8.0

4

17.5

11.5

13.0

17.0

15.0

8.0

5

16.5

8.5

12.5

17.0

14.5

8.0

6

17.0

11.0

12.5

17.0

14.5

9.0

7

17.5

10.0

15.0

15.5

14.5

8.0

8

19.5

10.0

13.0

18.5

15.0

8.0

9

15.5

9.5

14.0

16.0

16.0

9.0

10

16.0

10.0

13.0

16.5

14.5

9.0

Mean /min

16.7

10.35

13.15

16.6

15.0

8.3

SD/min

1.25

0.94

0.85

0.91

0.53

0.48

CV/%

7.50

9.12

6.48

5.46

3.51

5.82

Weak positive

1

20.5

32

51.5

38.5

20.5

40

2

21.5

29

53

38

23

37.5

3

23.5

34

51.5

39

20

34

4

22

35.5

51.5

43

22.5

29.5

5

20.5

28.5

48

38

23

33.5

6

22

31.5

49

39.5

20.5

31

7

24

33.5

47

39.5

19.5

35.5

8

21.5

29.5

45.5

48

19.5

37.5

9

21

33

42.5

33

20.5

34.5

10

21

35.5

48.5

39.5

18.5

30.5

Mean/min

21.75

32.2

48.8

39.6

20.75

34.35

SD/min

1.18

2.56

3.23

3.84

1.57

3.39

CV/%

5.45

7.96

6.63

9.69

7.56

9.87

  1. Medium positive, 30 times of the sensitivity. Weak positive, three times of the sensitivity. SD Standard deviation, CV Coefficient of variation, PQC Positive quality control, Streptococcus agalactiae (GBS), Enterococcus faecalis (EF), Gardnerella vaginalis (GV), Candida albicans (CA), Chlamydia trachomatis (CT)