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Table 1 Microbial rapid test (MBS) incubation temperature and test schedule

From: The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method

Strainsa

TVC

COLI

E.coli

S. aureus

Sal

Listeria

Incubation temperature (℃)

30

37

37

37

37

37

-

BLUE

RED

RED

RED

RED

BLUE

 + 

YELLOW

YELLOW

YELLOW

YELLOW

YELLOW

YELLOW

bacterial concentration

CFU/ml or CFU/g

Discoloration time(h)

1 × 107

< 3.0

< 4.0

< 4.3

< 7.3

< 4.0

< 7.3

1 × 106

3.0

4.0

4.3

7.3

4.0

7.3

1 × 105

5.3

6.3

7.3

11.0

4.3

12.0

1 × 104

8.0

9.3

10.0

20.0

8.3

16.3

1 × 103

11.0

12.3

13.0

29.3

13.3

20.3

1 × 102

14.0

16.0

16.0

36.0

18.0

25.0

1 × 101

16.0

19.0

19.0

43.0

23.0

29.0

1

18.0

22.0

22.0

46.0

28.0

33.0

0

> 20.0

> 24.0

> 24.0

> 48.0

> 32.0

> 36.0

  1. aE. coli Escherichia coli, Sal, Salmonella, S. aureus Staphylococcus aureus, Listeria Listeria monocytogenes, COLI Coliforms, TVC Total viable count