Fig. 7

Testing whether flaA and flaB can be simultaneously targeted by a single sgRNA in M1Cam and 81–176. Eight sgRNA target locations were identified in M1Cam and 81–176 flaA and flaB, whereby the red arrow represents shared sequence identity between flaA and flaB (A). Motility plating of CRISPRi transformants targeting flaA and flaB showed a significant motility decrease in M1Cam flaAB_428, flaAB_441, flaAB_527, flaAB_851 and flaAB_103, and 81–176 flaAB_428 and flaAB_1034 (B) (* = p < 0.05, ** = p < 0.01 and *** = p < 0.001, One-way T-test, N = 3 biological replicates, error bars = SD)